{ "items" : [ { "id" : "http://taxref.mnhn.fr/lod/bib/438479" "properties" : { "http://www.w3.org/1999/02/22-rdf-syntax-ns#type" : [ "http://schema.org/CreativeWork", "http://purl.org/ontology/bibo/Document" ] , "http://www.w3.org/2000/01/rdf-schema#label" : [ "Laidoudi et al. (2020)" ] , "http://www.w3.org/2000/01/rdf-schema#isDefinedBy" : [ "http://taxref.mnhn.fr/lod/taxref-ld" ] , "http://schema.org/datePublished" : [ "2020" ] , "http://purl.org/dc/terms/title" : [ "Laidoudi et al. (2020)" ] , "http://xmlns.com/foaf/0.1/page" : [ "https://www.mdpi.com/2076-2607/8/5/770/pdf" ] , "http://schema.org/identifier" : [ "nodeID://b70018565" ] , "http://purl.org/dc/terms/abstract" : [ "In French Guiana, canine heartworm disease is well known, but the diversity of filarial parasites of dogs remains largely unknown. A total of 98 canine blood samples from Cayenne and Kourou were assessed by a blood wet mount preparation, heartworm antigen test and molecular exploration of filarioid and Wolbachia DNAs, followed by a multiplex species-specific qPCR’s identification and a subsequent sequencing analysis. Thereafter, a phylogeny based on maximum likelihood was carried out to facilitate specific identification. Five dogs were microfilaremic. Heartworm antigens were detected in 15 (15.3%) dogs. Of these, six (6.1%) were considered as occult infections as neither microfilariae nor Dirofilaria immitis DNA were detected. The 11 (11.2%) D. immitis isolates corresponded to a low virulent strain. Six of the D. immitis isolates were positive for Wolbachia endosymbionts of D. immitis belonging to the clade C DNA. Acanthocheilonema reconditum DNA was detected in 3 (3.1%) samples. Of these latter, one was found co-infected with the Brugia sp. genotype and the DNA of the clade D of the Wolbachia endosymbiont of Brugia species. This latter was also detected in two filarioid DNA-free samples. Finally, two samples were positive for Cercopithifilaria bainae genotype, which is distinct from those identified in Europe. The present study highlights the urgent need to implement chemoprophylaxis associated with anti-Wolbachia drugs to control these potential zoonoses." ] , "http://purl.org/ontology/bibo/abstract" : [ "In French Guiana, canine heartworm disease is well known, but the diversity of filarial parasites of dogs remains largely unknown. A total of 98 canine blood samples from Cayenne and Kourou were assessed by a blood wet mount preparation, heartworm antigen test and molecular exploration of filarioid and Wolbachia DNAs, followed by a multiplex species-specific qPCR’s identification and a subsequent sequencing analysis. Thereafter, a phylogeny based on maximum likelihood was carried out to facilitate specific identification. Five dogs were microfilaremic. Heartworm antigens were detected in 15 (15.3%) dogs. Of these, six (6.1%) were considered as occult infections as neither microfilariae nor Dirofilaria immitis DNA were detected. The 11 (11.2%) D. immitis isolates corresponded to a low virulent strain. Six of the D. immitis isolates were positive for Wolbachia endosymbionts of D. immitis belonging to the clade C DNA. Acanthocheilonema reconditum DNA was detected in 3 (3.1%) samples. Of these latter, one was found co-infected with the Brugia sp. genotype and the DNA of the clade D of the Wolbachia endosymbiont of Brugia species. This latter was also detected in two filarioid DNA-free samples. Finally, two samples were positive for Cercopithifilaria bainae genotype, which is distinct from those identified in Europe. The present study highlights the urgent need to implement chemoprophylaxis associated with anti-Wolbachia drugs to control these potential zoonoses." ] , "http://purl.org/dc/terms/issued" : [ "2020" ] , "http://purl.org/dc/terms/bibliographicCitation" : [ "Laidoudi, Y., Marié, J., Tahir, D., Watier-Grillot, S., Mediannikov, O. & Davoust, B. 2020. Detection of Canine Vector-Borne Filariasis and Their Wolbachia Endosymbionts in French Guiana. Microorganisms, 8(5): 770." ] , "http://purl.org/ontology/bibo/doi" : [ "10.3390/microorganisms8050770" ] , "http://schema.org/sameAs" : [ "https://www.mdpi.com/2076-2607/8/5/770/pdf" ] } }, { "id" : "_:b70018565" "properties" : { "http://www.w3.org/1999/02/22-rdf-syntax-ns#type" : [ "http://schema.org/PropertyValue" ] , "http://schema.org/propertyID" : [ "http://www.wikidata.org/prop/direct/P356" ] , "http://schema.org/value" : [ "10.3390/microorganisms8050770"] } } }