This HTML5 document contains 17 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Subject Item

n2:438247

rdf:type

schema:CreativeWork bibo:Document

rdfs:label

Beaumelle <i>et al.</i> (2021)

rdfs:isDefinedBy

taxref:taxref-ld

schema:datePublished

2021-01-01

dct:title

Beaumelle <i>et al.</i> (2021)

foaf:page

n6:pdf

schema:identifier

_:vb70015352

dct:abstract

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Gastrointestinal nematodes are ubiquitous for both domestic and wild ungulates and have varying consequences for health and fitness. They exist as complex communities of multiple co-infecting species, and we have a limited understanding of how these communities vary in different hosts, regions and circumstances or of how this affects their impacts.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We have undertaken ITS2 rDNA nemabiome metabarcoding with next-generation sequencing on populations of nematode larvae isolated from 149 fecal samples of roe deer of different sex and age classes in the two isolated populations of Chizé and Trois Fontaines in France not co-grazing with any domestic ungulate species.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We identified 100 amplified sequence variants (ASVs) that were assigned to 14 gastrointestinal nematode taxa overall at either genus (29%) or species (71%) level. These taxa were dominated by parasites classically found in cervids—e.g.<jats:italic>Ostertagia leptospicularis</jats:italic>,<jats:italic>Spiculopteragia</jats:italic>spp. Higher parasite species diversity was present in the Trois Fontaines population than in the Chizé population including the presence of species more typically seen in domestic livestock (<jats:italic>Haemonchus contortus</jats:italic>,<jats:italic>Bunostomum</jats:italic>sp.,<jats:italic>Cooperia punctata</jats:italic>,<jats:italic>Teladorsagia circumcincta</jats:italic>). No differences in parasite species diversity or community composition were seen in the samples collected from three zones of differing habitat quality within the Chizé study area. Young roe deer hosted the highest diversity of gastrointestinal nematodes, with more pronounced effects of age apparent in Trois Fontaines. The effect of host age differed between gastrointestinal nematode species, e.g. there was little effect on<jats:italic>O. leptospicularis</jats:italic>but a large effect on<jats:italic>Trichostrongylus</jats:italic>spp. No effect of host sex was detected in either site.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The presence of some livestock parasite species in the Trois Fontaines roe deer population was unexpected given the isolation of this population away from grazing domestic livestock since decades. Overall, our results illustrate the influence of host traits and the local environment on roe deer nemabiome and demonstrate the power of the nemabiome metabarcoding approach to elucidate the composition of gastrointestinal nematode communities in wildlife.</jats:p></jats:sec><jats:sec><jats:title>Graphical Abstract</jats:title></jats:sec>

bibo:abstract

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Gastrointestinal nematodes are ubiquitous for both domestic and wild ungulates and have varying consequences for health and fitness. They exist as complex communities of multiple co-infecting species, and we have a limited understanding of how these communities vary in different hosts, regions and circumstances or of how this affects their impacts.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We have undertaken ITS2 rDNA nemabiome metabarcoding with next-generation sequencing on populations of nematode larvae isolated from 149 fecal samples of roe deer of different sex and age classes in the two isolated populations of Chizé and Trois Fontaines in France not co-grazing with any domestic ungulate species.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>We identified 100 amplified sequence variants (ASVs) that were assigned to 14 gastrointestinal nematode taxa overall at either genus (29%) or species (71%) level. These taxa were dominated by parasites classically found in cervids—e.g.<jats:italic>Ostertagia leptospicularis</jats:italic>,<jats:italic>Spiculopteragia</jats:italic>spp. Higher parasite species diversity was present in the Trois Fontaines population than in the Chizé population including the presence of species more typically seen in domestic livestock (<jats:italic>Haemonchus contortus</jats:italic>,<jats:italic>Bunostomum</jats:italic>sp.,<jats:italic>Cooperia punctata</jats:italic>,<jats:italic>Teladorsagia circumcincta</jats:italic>). No differences in parasite species diversity or community composition were seen in the samples collected from three zones of differing habitat quality within the Chizé study area. Young roe deer hosted the highest diversity of gastrointestinal nematodes, with more pronounced effects of age apparent in Trois Fontaines. The effect of host age differed between gastrointestinal nematode species, e.g. there was little effect on<jats:italic>O. leptospicularis</jats:italic>but a large effect on<jats:italic>Trichostrongylus</jats:italic>spp. No effect of host sex was detected in either site.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The presence of some livestock parasite species in the Trois Fontaines roe deer population was unexpected given the isolation of this population away from grazing domestic livestock since decades. Overall, our results illustrate the influence of host traits and the local environment on roe deer nemabiome and demonstrate the power of the nemabiome metabarcoding approach to elucidate the composition of gastrointestinal nematode communities in wildlife.</jats:p></jats:sec><jats:sec><jats:title>Graphical Abstract</jats:title></jats:sec>

dct:issued

2021-01-01

dct:bibliographicCitation

Beaumelle, C., Redman, E., De Rijke, J., Wit, J., Benabed, S., Debias, F., Duhayer, J., Pardonnet, S., Poirel, M., Capron, G., Chabot, S., Rey, B., Yannic, G., Gilleard, J. &amp; Bourgoin, G. 2021. Metabarcoding in two isolated populations of wild roe deer (<em>Capreolus capreolus</em>) reveals variation in gastrointestinal nematode community composition between regions and among age classes. <em>Parasites &amp; Vectors</em>, 14(1).

bibo:doi

10.1186/s13071-021-05087-5

schema:sameAs

n6:pdf

Subject Item

_:vb70015352

rdf:type

schema:PropertyValue

schema:propertyID

n7:P356

schema:value

10.1186/s13071-021-05087-5