This HTML5 document contains 17 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Subject Item

n2:439203

rdf:type

bibo:Document schema:CreativeWork

rdfs:label

Farnet <i>et al.</i> (1999)

rdfs:isDefinedBy

taxref:taxref-ld

schema:datePublished

1999-01-01

dct:title

Farnet <i>et al.</i> (1999)

foaf:page

n7:b99-051

schema:identifier

_:vb70015206

dct:abstract

<jats:p>Marasmius quercophilus is a white-rot basidiomycete isolated from evergreen oak litter. This fungus plays an extensive role in litter degradation because of its cellulase, pectinase, and laccase production. Our objective was to investigate the potential genotypic polymorphism of this species using RAPD (random amplified polymorphic DNA) and the amplification and the sequencing of fragments ITS (internal transcribed spacer) from the rRNA repeat. An intraspecies polymorphism was observed for the M. quercophilus isolates studied as suggested by the tree found with RAPD data: seven subgroups were defined. The minimum similarity coefficient observed in these groups was 0.278. The sizes of fragments ITS 1 and ITS 2 were the same for each isolate (ITS 1: 320 bp and ITS 2: 450 bp), and the percentages of nucleotide variation were 1.25 and 1.11%, respectively. Few isolates from the studied population had the same RAPD pattern that would suggest a clonal development in some zones of the site of La Gardiole.Key words: Marasmius quercophilus, intraspecific variability, ITS, RAPD.</jats:p>

bibo:abstract

<jats:p>Marasmius quercophilus is a white-rot basidiomycete isolated from evergreen oak litter. This fungus plays an extensive role in litter degradation because of its cellulase, pectinase, and laccase production. Our objective was to investigate the potential genotypic polymorphism of this species using RAPD (random amplified polymorphic DNA) and the amplification and the sequencing of fragments ITS (internal transcribed spacer) from the rRNA repeat. An intraspecies polymorphism was observed for the M. quercophilus isolates studied as suggested by the tree found with RAPD data: seven subgroups were defined. The minimum similarity coefficient observed in these groups was 0.278. The sizes of fragments ITS 1 and ITS 2 were the same for each isolate (ITS 1: 320 bp and ITS 2: 450 bp), and the percentages of nucleotide variation were 1.25 and 1.11%, respectively. Few isolates from the studied population had the same RAPD pattern that would suggest a clonal development in some zones of the site of La Gardiole.Key words: Marasmius quercophilus, intraspecific variability, ITS, RAPD.</jats:p>

dct:issued

1999-01-01

dct:bibliographicCitation

Farnet, A., Roux, M. &amp; Petit, J. 1999. Genotypic variations among isolates of <em>Marasmius quercophilus</em>, a white-rot fungus isolated from evergreen oak litter. <em>Canadian Journal of Botany</em>, 77(6): 884-890.

bibo:doi

10.1139/b99-051

schema:sameAs

n7:b99-051

Subject Item

_:vb70015206

rdf:type

schema:PropertyValue

schema:propertyID

n5:P356

schema:value

10.1139/b99-051